Genome-wide plant responses during the non-host interaction of tobacco with the Hemibiotrophic Bacterium Xanthomonas campestris pv. Vesicatoria

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dc.creator Pierella Karlusich, Juan J.
dc.creator Matias D. Zurbriggen, Matias D.
dc.creator Shahinnia, Fahimeh
dc.creator Sonnewald, Sophia
dc.creator Sonnewald, Uwe
dc.creator Hosseini, Seyed A.
dc.creator Hajirezaei, Mohammad-Reza
dc.creator Carrillo, Néstor
dc.date.accessioned 2021-02-19T23:05:59Z
dc.date.available 2021-02-19T23:05:59Z
dc.date.issued 2017-07-04
dc.identifier.issn 1664-462X es
dc.identifier.uri http://hdl.handle.net/2133/19660
dc.description Non-host resistance is the most ample and durable form of plant resistance against pathogen infection. It includes induction of defense-associated genes, massive metabolic reprogramming, and in many instances, a form of localized cell death (LCD) at the site of infection, purportedly designed to limit the spread of biotrophic and hemibiotrophic microorganisms. Reactive oxygen species (ROS) have been proposed to act as signals for LCD orchestration. They are produced in various cellular compartments including chloroplasts, mitochondria and apoplast. We have previously reported that down-regulation of ROS build-up in chloroplasts by expression of a plastid-targeted flavodoxin (Fld) suppressed LCD in tobacco leaves inoculated with the non-host bacterium Xanthomonas campestris pv. vesicatoria (Xcv), while other defensive responses were unaffected, suggesting that chloroplast ROS and/or redox status play a major role in the progress of LCD. To better understand these effects, we compare here the transcriptomic alterations caused by Xcv inoculation on leaves of Fld-expressing tobacco plants and their wild-type siblings. About 29% of leaf-expressed genes were affected by Xcv and/or Fld. Surprisingly, 5.8% of them (1,111 genes) were regulated by Fld in the absence of infection, presumably representing pathways responsive to chloroplast ROS production and/or redox status during normal growth conditions. While the majority (∼75%) of pathogen-responsive genes were not affected by Fld, many Xcv responses were exacerbated, attenuated, or regulated in opposite direction by expression of this protein. Particularly interesting was a group of 384 genes displaying Xcv responses that were already triggered by Fld in the absence of infection, suggesting that the transgenic plants had a larger and more diversified suite of constitutive defenses against the attacking microorganism compared to the wild type. Fld modulated many genes involved in pathogenesis, signal transduction, transcriptional regulation and hormone-based pathways. Remarkable interactions with proteasomal protein degradation were observed. The results provide the first genome-wide, comprehensive picture illustrating the relevance of chloroplast redox status in biotic stress responses. es
dc.description.sponsorship Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT): PICT 2012-2851 es
dc.description.sponsorship Deutscher Akademischer Austauschdienst (DAAD) es
dc.description.sponsorship European Molecular Biology Organization (EMBO) es
dc.description.sponsorship Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) es
dc.description.sponsorship Bunge & Born Foundation es
dc.description.sponsorship e Leibniz institute of Plant Genetics and Crop Plant Research es
dc.format application/pdf
dc.format.extent 1-21 es
dc.language.iso eng es
dc.publisher Frontiers Media es
dc.rights openAccess es
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ *
dc.subject Plant-microbe Interactions es
dc.subject Chloroplast Redox Status es
dc.subject Reactive Oxygen Species es
dc.subject Transcriptomics es
dc.subject Flavodoxin es
dc.subject Localized Cell Death es
dc.title Genome-wide plant responses during the non-host interaction of tobacco with the Hemibiotrophic Bacterium Xanthomonas campestris pv. Vesicatoria es
dc.type article
dc.type artículo
dc.type publishedVersion
dc.rights.holder Universidad Nacional de Rosario es
dc.rights.holder Pierella Karlusich, Juan J. es
dc.rights.holder Zurbriggen, Matias D. es
dc.rights.holder Shahinnia, Fahimeh es
dc.rights.holder Sonnewald, Sophia es
dc.rights.holder Sonnewald, Uwe es
dc.rights.holder Hosseini, Seyed A. es
dc.rights.holder Hajirezaei, Mohammad-Reza es
dc.rights.holder Carrillo, Néstor es
dc.relation.publisherversion https://doi.org/10.3389/fpls.2017.01158 es
dc.relation.publisherversion https://www.frontiersin.org/articles/10.3389/fpls.2017.01158/full es
dc.rights.text Attribution 4.0 International (CC BY 4.0) es
dc.citation.title Frontiers in Plant Science es
dc.citation.volume 8 es
dc.description.fil Fil: Pierella Karlusich, Juan J. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina. es
dc.description.fil Fil: Zurbriggen, Matias D. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina. es
dc.description.fil Fil: Shahinnia, Fahimeh. Leibniz Institute of Plant Genetics and Crop Plant Research; Germany. es
dc.description.fil Fil: Sonnewald, Sophia. Friedrich-Alexander-University Erlangen-Nuremberg. Division of Biochemistry. Department of Biology; Germany. es
dc.description.fil Fil: Sonnewald, Uwe. Friedrich-Alexander-University Erlangen-Nuremberg. Division of Biochemistry. Department of Biology; Germany. es
dc.description.fil Fil: Hosseini, Seyed A. Leibniz Institute of Plant Genetics and Crop Plant Research; Germany. es
dc.description.fil Fil: Hajirezaei, Mohammad-Reza. Leibniz Institute of Plant Genetics and Crop Plant Research; Germany. es
dc.description.fil Fil: Carrillo, Néstor. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina. es
dc.type.collection articulo
dc.type.version publishedVersion es


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