The histone-like protein HupB influences biofilm formation and virulence in Xanthomonas citri ssp. citri through the regulation of flagellar biosynthesis

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dc.creator Conforte, Valeria P.
dc.creator Malamud, Florencia
dc.creator Yaryura, Pablo M.
dc.creator Toum Terrones, Laila
dc.creator Torres, Pablo S.
dc.creator De Pino, Verónica
dc.creator Chazarreta, Cristian N.
dc.creator Gudesblat, Gustavo E.
dc.creator Castagnaro, Atilio P.
dc.creator Marano, María Rosa
dc.creator Vojnov, Adrián A.
dc.date.accessioned 2021-03-15T17:05:34Z
dc.date.available 2021-03-15T17:05:34Z
dc.date.issued 2018-12-08
dc.identifier.issn 1364-3703
dc.identifier.uri http://hdl.handle.net/2133/20156
dc.description Citrus canker is an important disease of citrus, whose causal agent is the bacterium Xanthomonas citri ssp. citri (Xcc). In previous studies, we found a group of Xcc mutants, generated by the insertion of the Tn5 transposon, which showed impaired ability to attach to an abiotic substrate. One of these mutants carries the Tn5 insertion in hupB, a gene encoding a bacterial histone-like protein, homologue to the β-subunit of the Heat-Unstable (HU) nucleoid protein of Escherichia coli. These types of protein are necessary to maintain the bacterial nucleoid organization and the global regulation of gene expression. Here, we characterized the influence of the mutation in hupB regarding Xcc biofilm forma tion and virulence. The mutant strain hupB was incapable of swimming in soft agar, whereas its complemented strain partially recovered this phenotype. Electron microscope imaging revealed that impaired motility of hupB was a consequence of the absence of the flagellum. Comparison of the expression of flagellar genes between the wild-type strain and hupB showed that the mutant exhibited decreased expression of fliC (encoding flagellin). The hupB mutant also displayed reduced virulence compared with the wild-type strain when they were used to infect Citrus lemon plants using different infection methods. Our results therefore show that the histone-like protein HupB plays an essential role in the pathogenesis of Xcc through the regulation of biofilm formation and biosynthesis of the flagellum. es
dc.description.sponsorship Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT) es
dc.description.sponsorship Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) es
dc.format application/pdf
dc.format.extent 589–598
dc.language.iso eng es
dc.publisher Wiley Open Access es
dc.rights openAccess es
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ *
dc.subject Biofilms es
dc.subject Citrus es
dc.subject Flagella es
dc.subject Histone-Like Protein es
dc.subject Virulence es
dc.subject Xanthomonas es
dc.title The histone-like protein HupB influences biofilm formation and virulence in Xanthomonas citri ssp. citri through the regulation of flagellar biosynthesis es
dc.type publishedVersion
dc.rights.holder Universidad Nacional de Rosario es
dc.rights.holder Conforte, Valeria P. es
dc.rights.holder Malamud, Florencia es
dc.rights.holder Yaryura, Pablo M. es
dc.rights.holder Toum Terrones, Laila es
dc.rights.holder Torres, Pablo S. es
dc.rights.holder De Pino, Verónica es
dc.rights.holder Chazarreta, Cristian N. es
dc.rights.holder Gudesblat, Gustavo E. es
dc.rights.holder Castagnaro, Atilio P. es
dc.rights.holder Marano, María Rosa. es
dc.rights.holder Vojnov, Adrián A. es
dc.relation.publisherversion https://doi.org/10.1111/mpp.12777 es
dc.relation.publisherversion https://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1111/mpp.12777 es
dc.rights.text Attribution 4.0 International (CC BY 4.0) es
dc.citation.title Molecular Plant Pathology
dc.citation.volume 20(4)
dc.description.fil Fil: Conforte, Valeria P. Instituto de Ciencia y Tecnología Dr. César Milstein (ICT – CONICET - Fundación Pablo Cassará); Argentina.
dc.description.fil Fil: Malamud, Florencia. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina.
dc.description.fil Fil: Yaryura, Pablo M. Universidad de Villa María. Centro de Investigaciones y Transferencia de Villa María (CIT - CONICET); Argentina.
dc.description.fil Fil: Toum Terrones, Laila. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina.
dc.description.fil Fil: Toum Terrones, Laila. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA - CONICET); Argentina.
dc.description.fil Fil: Torres, Pablo S. Instituto de Ciencia y Tecnología Dr. César Milstein (ICT – CONICET - Fundación Pablo Cassará); Argentina.
dc.description.fil Fil: De Pino, Verónica. Instituto de Ciencia y Tecnología Dr. César Milstein (ICT – CONICET - Fundación Pablo Cassará); Argentina.
dc.description.fil Fil: Chazarreta, Cristian N. Instituto de Ciencia y Tecnología Dr. César Milstein (ICT – CONICET - Fundación Pablo Cassará); Argentina.
dc.description.fil Fil: Gudesblat, Gustavo E. Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA – CONICET -Estación Experimental Agroindustrial Obispo Colombres (EEAOC); Argentina.
dc.description.fil Fil: Castagnaro, Atilio P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina.
dc.description.fil Fil: Castagnaro, Atilio P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA - CONICET); Argentina.
dc.description.fil Fil: Marano, María Rosa. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina.
dc.description.fil Fil: Marano, María Rosa. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.
dc.description.fil Fil: Vojnov, Adrián A. Instituto de Ciencia y Tecnología Dr. César Milstein (ICT – CONICET - Fundación Pablo Cassará); Argentina.


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