Microevolution in the major outer membrane protein OmpA of Acinetobacter baumannii

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dc.creator Viale, Alejandro M.
dc.creator Evans, Benjamin A.
dc.date.accessioned 2021-04-20T00:14:54Z
dc.date.available 2021-04-20T00:14:54Z
dc.date.issued 2020-06-04
dc.identifier.issn 2057-5858
dc.identifier.uri http://hdl.handle.net/2133/20517
dc.description Acinetobacter baumannii is nowadays a relevant nosocomial pathogen characterized by multidrug resistance (MDR) and concomitant difficulties to treat infections. OmpA is the most abundant A. baumannii outer membrane (OM) protein, and is involved in virulence, host-cell recognition, biofilm formation, regulation of OM stability, permeability and antibiotic resistance. OmpA members are two‐domain proteins with an N‐terminal eight‐stranded β‐barrel domain with four external loops (ELs) interact ing with the environment, and a C‐terminal periplasmic domain binding non‐covalently to the peptidoglycan. Here, we combined data from genome sequencing, phylogenetic and multilocus sequence analyses from 975 strains/isolates of the Acinetobacter calcoaceticus/Acinetobacter baumannii complex (ACB), 946 from A. baumannii, to explore ompA microevolutionary divergence. Five major ompA variant groups were identified (V1 to V5) in A. baumannii, encompassing 52 different alleles coding for 23 different proteins. Polymorphisms were concentrated in five regions corresponding to the four ELs and the C‐terminal end, and provided evidence for intra‐genic recombination. ompA variants were not randomly distributed across the A. baumannii phylogeny, with the most frequent V1(lct)a1 allele found in most clonal complex 2 (CC2) strains and the second most frequent V2(lct)a1 allele in the majority of CC1 strains. Evidence was found for assortative exchanges of ompA alleles not only between separate A. baumannii lineages, but also different ACB species. The overall results have implications for A. baumannii evolution, epidemiology, virulence and vaccine design. es
dc.description.sponsorship Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT): PICT‐2015‐1072 es
dc.description.sponsorship Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) es
dc.description.sponsorship Ministerio de Salud de Santa Fe es
dc.format application/pdf
dc.format.extent 1-14
dc.language.iso eng es
dc.publisher Microbiology Society es
dc.rights openAccess es
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ *
dc.subject Acinetobacter baumannii es
dc.subject Protein Evolution es
dc.subject OmpA es
dc.subject Recombination es
dc.title Microevolution in the major outer membrane protein OmpA of Acinetobacter baumannii es
dc.type publishedVersion
dc.rights.holder Universidad Nacional de Rosario es
dc.rights.holder Viale, Alejandro M. es
dc.rights.holder Evans, Benjamin A. es
dc.relation.publisherversion https://doi.org/10.1099/mgen.0.000381 es
dc.relation.publisherversion https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000381 es
dc.rights.text Attribution 4.0 International (CC BY 4.0) es
dc.citation.title Microbial Genomics
dc.citation.volume 6(6)
dc.description.fil Fil: Viale, Alejandro M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.
dc.description.fil Fil: Viale, Alejandro M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina.
dc.description.fil Fil: Evans, Benjamin A. University of East Anglia. Norwich Medical School; United Kingdom.


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